Use of continuous-elution gel electrophoresis as a preparative tool for blot overlay analysis
نویسندگان
چکیده
منابع مشابه
Continuous preparative gel electrophoresis.
Electrophoresis is widely used as a separative technique, particularly in protein chemistry. In some respects gelatinous media have an advantage over others as support media for electrophoresis, perhaps most obviously when a gel matrix of molecular dimensions is employed, allowing the simultaneous use of two properties for separation of the particles of interest-size and surface charge density....
متن کاملA new apparatus for preparative gel electrophoresis.
paratus is the dimension of the elution cham ber; if a large chamber is employed, dilution of solute occurs thus resulting in broadening of the elution patterns, while with a narrow chamber elution is frequently interrupted by the swelling of gel during operation. In many cases, either upward or downward elution has been employed and this leads to com plication of the apparatus. This communi ca...
متن کاملUse of capillary electrophoresis as a method development tool for classical gel electrophoresis.
Capillary electrophoresis (CE) was used to optimize the buffer pH, ionic strength and sulfated cyclodextrin concentrations for enantiomeric separation of piperoxan. These enantioseparation conditions were then applied to a classical gel electrophoresis system. Binding constants of the sulfated beta-cyclodextrin-piperoxan couple were approximated using CE and the effects of organic solvents on t...
متن کاملcomparative analysis of denaturing gradient gel electrophoresis and temporal temperature gradient gel electrophoresis profiles as a tool for the differentiation of candida species
results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...
متن کاملOnline tool for analysis of denaturing gradient gel electrophoresis profiles.
We present an online tool (EquiBands, http://www.univie.ac.at/IECB/limno/equibands/EquiBands.html) that quantifies the matching of two bands considered to be the same in different samples, even when samples are applied to different denaturing gradient gel electrophoresis gels. With an environmental example we demonstrate the procedure for the classification of two bands of different samples wit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2003
ISSN: 0003-2697
DOI: 10.1016/s0003-2697(03)00321-x